The chick embryo as an effective animal model in testing the toxicological effects of snake venom
Basic information
Slovak title:
Kuracie embryo ako efektívny zvierací model v testovaní toxikologických účinkov hadieho jedu
Registration No.:
1/0241/18
Source:
Scientific grant agency (Ministry of education, science, research and sport of the Slovak Republic)
Implementation time:
2018 - 2021
Principal (UVMP):
Petrilla Vladimír, MVDr., PhD. (2018: 14.75% | 2019: 14.06% | 2020: 13.64% | 2021: 13.64%)
Principal workplace:
Department of Biology and Physiology
Principal workplace:
Department of Biology and Physiology
Contributors:
Falis Marcel, doc. MVDr., PhD. (2018: 8.2% | 2019: 7.81% | 2020: 7.58% | 2021: 7.58%)
Petrillová Monika, Mgr. (2018: 16.39% | 2019: 15.63% | 2020: 15.15% | 2021: 15.15%)
Andrejčáková Zuzana, MVDr., PhD. (2018: 11.48% | 2019: 10.94% | 2020: 10.61% | 2021: 10.61%)
Marcinčáková Dana, doc. MVDr., PhD. (2018: 4.92% | 2019: 4.69% | 2020: 4.55% | 2021: 4.55%)
Ondrašovičová Silvia, MVDr., PhD. (2018: 4.92% | 2019: 4.69% | 2020: 3.03% | 2021: 5.06%)
Korim Peter, doc. MVDr., CSc. (2018: 8.2% | 2019: 7.81% | 2020: 7.58% | 2021: 7.58%)
Korimová Jana, Ing. MVDr., PhD. (2018: 8.2% | 2019: 7.81% | 2020: 7.58% | 2021: 7.58%)
Takáč Ladislav, Ing., PhD., LL.M, MPA, DBA (2018: 3.28% | 2019: 7.81% | 2020: 3.03% | 2021: 3.03%)
Bartková Valéria, PhDr. (2018: 16.38% | 2019: 15.63% | 2020: 15.15% | 2021: 14.12%)
Eibenová Andrea, Mgr. (2018: 3.28% | 2019: 3.12% | 2020: 12.1% | 2021: 11.1%)
Field of study:
200 - Veterinary Sciences
Financial indicators
NON-CAPITAL FUNDING in €
CAPITAL FUNDING in €
Allocated
Spent
Allocated
Spent
Allocated / Spent
Allocated / Spent
2018
14020
14020
0
0
14020 / 14020
0 / 0
2019
14235
14235
0
0
14235 / 14235
0 / 0
2020
15013
15013
0
0
15013 / 15013
0 / 0
2021
15262
15262
0
0
15262 / 15262
0 / 0
Spolu
58530
58530
0
0
58530 / 58530
0 / 0
Anotation
Projekt bude sledovať vývinovú toxicitu kuracích embryí po aplikácii hadích jedov prejavujúcich sa významne pri
vývine nervového a kardiovaskulárneho systému ako aj vývine pečene, ktorá sa významnou úlohou podieľa na
biotransformácii a vylučovaní toxínov z tela jedinca. Aplikuje sa injekcia o príslušnej koncentrácii hadieho jedu na
membrana papyracea, čím dôjde k rovnomernému rozloženiu toxínu vo vajci. Pre porovnanie výsledkov, v
nasledujúcej fáze projektu bude vykonaná intraamniálna aplikácia hadieho jedu. V projekte budú využité
biochemické, histologické, imunohistochemické, kvantitatívne kolorimetrické a morfometrické metódy, ktoré
vzájomným sofistikovaným dopĺňaním zabezpečia vierohodné výsledky. Závery projektu budú dôležité pre
následné epidemiologické štúdie vývinovej neurotoxicity a cytotoxicity u ľudí.
The aim of the project will be monitoring of the developmental toxicity of the chick embryos after snake venom
injection, expressed specifically during the development of the nervous and cardiovascular system and also
during the liver development, which plays a major role in biotransformation and excretion of toxins from the body.
The injection of the relevant concentration of venom will be applied on membrane papyracea, which receives an
equal distribution of toxins in an egg. For comparison purposes of the results, the following phase of the project
will be aimed at intraamnial application. To achieve the trustworthy results of the project, biochemical,
histological, immunohistochemical, quantitative colorimetric methods, and morphometric methods will be used to
provide mutually complementing sophisticated, reliable results. The conclusions of the project will be important for
subsequent epidemiological studies of developmental neurotoxicity and cytotoxicity in humans.
Publication outputs